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mouse anti blvrb (Santa Cruz Biotechnology)

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Santa Cruz Biotechnology mouse anti blvrb
Mouse Anti Blvrb, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-blvrb/BLVRB+Antibody/pm40216753-378-77-79
Average 93 stars, based on 6 article reviews

mouse anti blvrb - by Bioz Stars, 2026-10

93/100 stars

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Article Title: Small molecule BLVRB redox inhibitor promotes megakaryocytopoiesis and stress thrombopoiesis in vivo
Article Snippet: .. Protein lysates were size-fractionated by SDS-PAGE , transferred to nitrocellulose membranes, and immunodetection was completed using sheep anti-BLVRB antibody (1:1000; R&D Systems; Minneapolis, MN cat#AF6568) followed by donkey anti-sheep antibody (1:2000; Jackson ImmunoResearch; West Grove, PA; cat#713-035-147); mouse anti-BLVRA (1:1000; Santa Cruz; Dallas, TX cat#sc-393385) followed by goat anti-mouse antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat#115-585-003); goat anti-Transferrin Receptor (CD71; R & D Systems, Minneapolis, MN cat#AF2474) followed by mouse anti-goat antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat#205-032-176); mouse anti-BlvrB (Santa Cruz; Dallas, TX cat#sc-373692) followed by goat anti-mouse (1:5000; Jackson ImmunoResearch; West Grove, PA cat#115-036-072); rabbit anti-PF4 (1:500; Abcam; Cambridge, UK cat#ab129183) followed by donkey anti-rabbit (1:5000; Millipore Sigma; Burlington, MA cat#MANA9340); rabbit anti-CD61 (1:1000; Proteintech; Rosement, IL cat#18309-1-AP) followed by donkey anti-rabbit (1:5000, Millipore Sigma, Burlington, MA cat#NA9340); mouse anti-actin antibody (1:1000; Millipore Sigma; Burlington, MA cat#MAB1501R) followed by goat anti-mouse antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat#115-036-072); mouse anti-GAPDH antibody (1:1000; Invitrogen; Carlsbad, CA cat#MA5-15738) followed by goat anti-mouse antibody (1:5000; Jackson ImmunoResearch; West Grove, PA cat#115-036-072). .. Immunodetection was completed by enhanced chemiluminescence using LuminataTM Forte Western HRP substrate (Millipore Sigma; Burlington, MA) and visualized using a C-DiGit® blot scanner (LI-COR; Lincoln, NE).

Immunodetection:

Article Title: Small molecule BLVRB redox inhibitor promotes megakaryocytopoiesis and stress thrombopoiesis in vivo.
Article Snippet: .. Protein lysates were size-fractionated by SDS-PAGE7, transferred to nitrocellulose membranes, and immunodetection was completed using sheep anti-BLVRB antibody (1:1000; R&D Systems; Minneapolis, MN cat#AF6568) followed by donkey anti-sheep antibody (1:2000; Jackson ImmunoResearch;West Grove, PA; cat#713-035-147);mouse anti-BLVRA (1:1000; Santa Cruz; Dallas, TX cat#sc-393385) followed by goat anti-mouse antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat#115585-003); goat anti-Transferrin Receptor (CD71; R & D Systems, Minneapolis, MN cat#AF2474) followed by mouse anti-goat antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat#205-032-176); mouse anti-BlvrB (Santa Cruz; Dallas, TX cat#sc-373692) followed by goat anti-mouse (1:5000; Jackson ImmunoResearch; West Grove, PA cat#115-036-072); rabbit anti-PF4 (1:500; Abcam; Cambridge, UK cat#ab129183) followed by donkey anti-rabbit (1:5000; Millipore Sigma; Burlington, MA cat#MANA9340); rabbit anti-CD61 (1:1000; Proteintech; Rosement, IL cat#18309-1-AP) followed by donkey antirabbit (1:5000, Millipore Sigma, Burlington, MA cat#NA9340); mouse anti-actin antibody (1:1000; Millipore Sigma; Burlington, MA cat#MAB1501R) followed by goat anti-mouse antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat#115-036-072); mouse anti- Nature Communications | (2025) 16:3480 14 GAPDH antibody (1:1000; Invitrogen; Carlsbad, CA cat#MA5-15738) followed by goat anti-mouse antibody (1:5000; Jackson ImmunoResearch; West Grove, PA cat#115-036-072). .. Immunodetection was completed by enhanced chemiluminescence using LuminataTM Forte Western HRP substrate (Millipore Sigma; Burlington, MA) and visualized using a C-DiGit® blot scanner (LI-COR; Lincoln, NE).

other:

Article Title: Methods to separate nuclear soluble fractions reflecting localizations in living cells
Article Snippet: Antibodies The antibodies used in this study were as follows: mAb414 ( Davis and Blobel, 1986 ) (902902; BioLegend) at 1:500 for IF, QE5 ( Panté et al., 1994 ) (ab24700; Abcam) at 1:100 for IF, RL2 ( Holt et al., 1987 ; Snow et al., 1987 ) (NB300-524; Novus biologicals) at 1:200 for IF, mouse anti-GAPDH (sc-32233; Santa Cruz Biotechnology) at 1:100 for IF and 1:1000 for WB, mouse anti-α-tubulin (T9026; Merck) at 1:500 for IF and 1:1000 for WB, goat anti-RanBP1 (ab17034; Abcam) at 1:200 for IF and 1:1000 for WB, mouse anti-Lamin A/C (sc-7292; Santa Cruz Biotechnology) at 1:200 for both IF and WB, rabbit anti-Histone H3 (ab1791; Abcam) at 1:200 for IF and 1:5000 for WB, goat anti-RCC1 (sc-1162; Santa Cruz Biotechnology) at 1:200 for both IF and WB, mouse anti-PCNA (610665; BD Biosciences) at 1:2000 for WB, mouse anti-PCNA (sc-56; Santa Cruz Biotechnology) at 1:50 for IF, rabbit anti-RanBP3 (NB100-74647; Novus biologicals) at 1:100 for IF and 1:1000 for WB, mouse anti- profilin 1 (PFN1) (67390-1-Ig; Proteintech) at 1:100 for IF and 1:2000 for WB, rabbit anti- thioredoxin (TXN) (14999-1-AP; Proteintech) at 1:50 for IF and 1:1000 for WB, rabbit anti-Calnexin (2679; Cell Signaling TECHNOLOGY) at 1:1000 for WB, rabbit anti-PDI (3501; Cell Signaling TECHNOLOGY) at 1:1000 for WB, rabbit anti-RCAS1 (12290; Cell Signaling TECHNOLOGY) at 1:1000 for WB, rabbit anti-HINT1 (10717-1-AP; Proteintech) at 1:100 for IF and 1:1000 for WB, mouse anti-ACP1 (sc-390190; Santa Cruz Biotechnology) at 1:100 for IF and 1:1000 for WB, mouse anti-BLVRB (sc-373692; Santa Cruz Biotechnology) at 1:50 for IF and 1:1000 for WB, and mouse anti-Ran (610341; BD Biosciences) at 1:500 for IF and 1:1000 for WB.

Western Blot:

Article Title: Methods to separate nuclear soluble fractions reflecting localizations in living cells
Article Snippet: To prepare a digitonin solution supplemented with WGA (digitonin + WGA solution) or succinylated WGA, 5 mg/mL biotinylated WGA solution (B-1025-5; VECTOR LABORATORIES) or 5 mg/mL biotinylated succinylated WGA solution (B-1025S-5; VECTOR LABORATORIES) was mixed in 100 μg/mL digitonin solution. .. The antibodies used in this study were as follows: mAb414 ( Davis and Blobel, 1986 ) (902902; BioLegend) at 1:500 for IF, QE5 ( Panté et al., 1994 ) (ab24700; Abcam) at 1:100 for IF, RL2 ( Holt et al., 1987 ; Snow et al., 1987 ) (NB300-524; Novus biologicals) at 1:200 for IF, mouse anti-GAPDH (sc-32233; Santa Cruz Biotechnology) at 1:100 for IF and 1:1000 for WB, mouse anti-α-tubulin (T9026; Merck) at 1:500 for IF and 1:1000 for WB, goat anti-RanBP1 (ab17034; Abcam) at 1:200 for IF and 1:1000 for WB, mouse anti-Lamin A/C (sc-7292; Santa Cruz Biotechnology) at 1:200 for both IF and WB, rabbit anti-Histone H3 (ab1791; Abcam) at 1:200 for IF and 1:5000 for WB, goat anti-RCC1 (sc-1162; Santa Cruz Biotechnology) at 1:200 for both IF and WB, mouse anti-PCNA (610665; BD Biosciences) at 1:2000 for WB, mouse anti-PCNA (sc-56; Santa Cruz Biotechnology) at 1:50 for IF, rabbit anti-RanBP3 (NB100-74647; Novus biologicals) at 1:100 for IF and 1:1000 for WB, mouse anti- profilin 1 (PFN1) (67390-1-Ig; Proteintech) at 1:100 for IF and 1:2000 for WB, rabbit anti- thioredoxin (TXN) (14999-1-AP; Proteintech) at 1:50 for IF and 1:1000 for WB, rabbit anti-Calnexin (2679; Cell Signaling TECHNOLOGY) at 1:1000 for WB, rabbit anti-PDI (3501; Cell Signaling TECHNOLOGY) at 1:1000 for WB, rabbit anti-RCAS1 (12290; Cell Signaling TECHNOLOGY) at 1:1000 for WB, rabbit anti-HINT1 (10717-1-AP; Proteintech) at 1:100 for IF and 1:1000 for WB, mouse anti-ACP1 (sc-390190; Santa Cruz Biotechnology) at 1:100 for IF and 1:1000 for WB, mouse anti-BLVRB (sc-373692; Santa Cruz Biotechnology) at 1:50 for IF and 1:1000 for WB, and mouse anti-Ran (610341; BD Biosciences) at 1:500 for IF and 1:1000 for WB. .. The secondary antibodies used for IF were as follows: donkey anti-rabbit IgG-Alexa Fluor 488 (ab150061; Abcam) at 1:500, goat anti-mouse IgG-Alexa Fluor 488 (A-11029; Thermo Fisher Scientific) at 1:500, and donkey anti-goat IgG-Cy2 (705-225-147; Jackson ImmunoResearch Laboratories) at 1:200.

Image Search Results

Elevated Nox-mediated oxidative stress and Tlr4/MyD88-mediated inflammation in lungs of mice with BLM-induced pulmonary fibrosis (PF). ( a ) Schematic diagram for the creation of the BLM-induced PF mouse model. C57BL/6 mice that were 6–8 weeks old were injured by intratracheal instillation of 1.5 mg/kg BLM at day 0 (d0) and d7, and the lung tissues were harvested at d14 post-injury for evaluation (N = 8). ( b ) Representative histochemical images of H&E and Masson staining demonstrated alveolar structural damage (H&E staining) with ECM deposition (Masson staining). ( c ) Representative immunoblotting blots demonstrated an increased expression of proteins of Nox family numbers (Nox2, Nox3, and Nox4), oxidization stress markers (Nrf2 and Ho-1), and EMT markers (α-SMA and Vimentin) in the lungs of BLM-induced PF mice compared with the saline group. ( d ) Semi-quantitation of relative levels of proteins of interest as determined by the relative density over Gapdh in ( c ). ( e ) IF staining validated the increased abundance of α-SMA in the lungs of mice at d14 post-BLM challenge compared with saline controls. ( f ) Representative blots of immunoblotting assay demonstrated the enhanced activation of Tlr4/MyD88 signaling in the lung of BLM-induced PF mice model compared with the saline group. ( g ) Semi-quantitation of the relative levels of proteins of interest Tlr4, MyD88, and p-NF-κB as evaluated by the relative density over Gapdh in ( f ). Data in ( d , g ) represent mean ± SD from the indicated number of mice (N = 3), as analyzed by GraphPad prism one-way ANOVA Tukey’s multiple comparisons test.

Journal: Biomedicines

Article Title: Secretome of hESC-Derived MSC-like Immune and Matrix Regulatory Cells Mitigate Pulmonary Fibrosis through Antioxidant and Anti-Inflammatory Effects

doi: 10.3390/biomedicines11020463

Figure Lengend Snippet: Elevated Nox-mediated oxidative stress and Tlr4/MyD88-mediated inflammation in lungs of mice with BLM-induced pulmonary fibrosis (PF). ( a ) Schematic diagram for the creation of the BLM-induced PF mouse model. C57BL/6 mice that were 6–8 weeks old were injured by intratracheal instillation of 1.5 mg/kg BLM at day 0 (d0) and d7, and the lung tissues were harvested at d14 post-injury for evaluation (N = 8). ( b ) Representative histochemical images of H&E and Masson staining demonstrated alveolar structural damage (H&E staining) with ECM deposition (Masson staining). ( c ) Representative immunoblotting blots demonstrated an increased expression of proteins of Nox family numbers (Nox2, Nox3, and Nox4), oxidization stress markers (Nrf2 and Ho-1), and EMT markers (α-SMA and Vimentin) in the lungs of BLM-induced PF mice compared with the saline group. ( d ) Semi-quantitation of relative levels of proteins of interest as determined by the relative density over Gapdh in ( c ). ( e ) IF staining validated the increased abundance of α-SMA in the lungs of mice at d14 post-BLM challenge compared with saline controls. ( f ) Representative blots of immunoblotting assay demonstrated the enhanced activation of Tlr4/MyD88 signaling in the lung of BLM-induced PF mice model compared with the saline group. ( g ) Semi-quantitation of the relative levels of proteins of interest Tlr4, MyD88, and p-NF-κB as evaluated by the relative density over Gapdh in ( f ). Data in ( d , g ) represent mean ± SD from the indicated number of mice (N = 3), as analyzed by GraphPad prism one-way ANOVA Tukey’s multiple comparisons test.

Article Snippet: Then, the sections were stained with 1:200 rabbit monoclonal IgG Heme oxygenase-1 (HO-1) (Proteintech, 27282-1-AP), rabbit monoclonal IgG nuclear factor erythroid 2–related factor 2 (NRF2) (Proteintech, 16396-1-AP), rabbit monoclonal IgG smooth muscle actin (SMA) (Proteintech, 14395-1-AP), rabbit monoclonal IgG NADPH Oxidase 4 (NOX4) (BOSTER, BA2813), rabbit monoclonal IgG Activating Transcription Factor 6 (ATF6) (Proteintech, 24169-1-AP), rabbit monoclonal IgG N-cadherin (Proteintech, 22018-1-AP), and mouse monoclonal IgG2b TNF-α (Proteintech, 60291-1-IG) overnight at 4 °C in 5% donkey serum in PBS.

Techniques: Staining, Western Blot, Expressing, Saline, Quantitation Assay, Activation Assay

BLM induces NOX4-mediated oxidative stress in A549 lung epithelial cells. ( a ) Representative images of immunoblotting blots revealed an increased expression of α-SMA, NOX4 and NRF2 in A549 cells exposed to indicated concentrations of BLM (1.0, 2.5, 5.0, 10.0, and 20.0 μg/mL) for 48 h. ( b ) Semi-quantitation of the relative levels of α-SMA, NOX4, and NRF2 proteins as evaluated by the relative density over GAPDH in ( a ). ( c ) Representative images of A549 cells treated with indicated concentrations of BLM for 48 h and stained with CellROX ® Orange reagent. The BLM-induced A549 injury increased ROS production, especially at the concentration of 5 μg/mL, compared to untreated cells (NC). ( d ) Mean gray value of ROS production in ( c ). ( e ) Representative images of IF exhibited more abundant HO-1, NRF2, NOX4, ATF6, α-SMA, and N-Cad proteins in A549 cells exposed to 5 μg/mL of BLM for 48 h as compared with the NC control. ( f ) Representative images of the fluorescent intensity of the relevant proteins in ( e ). Data in ( b , d , f ) represent mean ± SD from three independent experiments (N = 3), as analyzed by GraphPad prism one-way ANOVA Tukey’s multiple comparisons test. Scale bars in ( c ), 100 µm; ( e ), 10 µm.

Journal: Biomedicines

Article Title: Secretome of hESC-Derived MSC-like Immune and Matrix Regulatory Cells Mitigate Pulmonary Fibrosis through Antioxidant and Anti-Inflammatory Effects

doi: 10.3390/biomedicines11020463

Figure Lengend Snippet: BLM induces NOX4-mediated oxidative stress in A549 lung epithelial cells. ( a ) Representative images of immunoblotting blots revealed an increased expression of α-SMA, NOX4 and NRF2 in A549 cells exposed to indicated concentrations of BLM (1.0, 2.5, 5.0, 10.0, and 20.0 μg/mL) for 48 h. ( b ) Semi-quantitation of the relative levels of α-SMA, NOX4, and NRF2 proteins as evaluated by the relative density over GAPDH in ( a ). ( c ) Representative images of A549 cells treated with indicated concentrations of BLM for 48 h and stained with CellROX ® Orange reagent. The BLM-induced A549 injury increased ROS production, especially at the concentration of 5 μg/mL, compared to untreated cells (NC). ( d ) Mean gray value of ROS production in ( c ). ( e ) Representative images of IF exhibited more abundant HO-1, NRF2, NOX4, ATF6, α-SMA, and N-Cad proteins in A549 cells exposed to 5 μg/mL of BLM for 48 h as compared with the NC control. ( f ) Representative images of the fluorescent intensity of the relevant proteins in ( e ). Data in ( b , d , f ) represent mean ± SD from three independent experiments (N = 3), as analyzed by GraphPad prism one-way ANOVA Tukey’s multiple comparisons test. Scale bars in ( c ), 100 µm; ( e ), 10 µm.

Techniques: Western Blot, Expressing, Quantitation Assay, Staining, Concentration Assay, Control

hESC-MSC-IMRC-CM reduces the BLM-induced oxidative stress and EMT in A549 lung epithelial cells. ( a ) Representative immunoblots showed that hESC-MSC-IMRC-CM could reduce BLM-induced A549 cells oxidative injury by decreasing the expression of NRF2, HO-1, NOX4, and EMT markers’ N-Cad. ( b ) The relative levels of NRF2, HO-1, NOX4, and N-Cad proteins as evaluated by a densitometric analysis in ( a ). ( c ) Representative images of A549 cells treated with indicated conditions for 48 h and stained with CellROX ® Orange reagent. hESC-MSC-IMRC-CM significantly reduced the ROS production compared to the BLM group. ( d ) Mean gray value of ROS production in ( c ). ( e ) hESC-MSC-IMRC-CM increased glutathione peroxidase (GSH-PX) production in A549 cells compared to untreated controls. Data in ( b , d , e ) represent mean ± SD of three independent experiments (N = 3), as analyzed by GraphPad prism Two-way ANOVA Tukey’s multiple comparisons test. Scale bars in ( c ), 100 μm.

Journal: Biomedicines

Article Title: Secretome of hESC-Derived MSC-like Immune and Matrix Regulatory Cells Mitigate Pulmonary Fibrosis through Antioxidant and Anti-Inflammatory Effects

doi: 10.3390/biomedicines11020463

Figure Lengend Snippet: hESC-MSC-IMRC-CM reduces the BLM-induced oxidative stress and EMT in A549 lung epithelial cells. ( a ) Representative immunoblots showed that hESC-MSC-IMRC-CM could reduce BLM-induced A549 cells oxidative injury by decreasing the expression of NRF2, HO-1, NOX4, and EMT markers’ N-Cad. ( b ) The relative levels of NRF2, HO-1, NOX4, and N-Cad proteins as evaluated by a densitometric analysis in ( a ). ( c ) Representative images of A549 cells treated with indicated conditions for 48 h and stained with CellROX ® Orange reagent. hESC-MSC-IMRC-CM significantly reduced the ROS production compared to the BLM group. ( d ) Mean gray value of ROS production in ( c ). ( e ) hESC-MSC-IMRC-CM increased glutathione peroxidase (GSH-PX) production in A549 cells compared to untreated controls. Data in ( b , d , e ) represent mean ± SD of three independent experiments (N = 3), as analyzed by GraphPad prism Two-way ANOVA Tukey’s multiple comparisons test. Scale bars in ( c ), 100 μm.

Techniques: Western Blot, Expressing, Staining

hESC-MSC-IMRCs and hESC-MSC-IMRC-CM inhibit the development of pulmonary fibrosis in mice. ( a ) Schematic illustration shows the experimental workflow for the treatment of the BLM-induced IPF mouse model at an early stage of PF development. C57BL/6 mice were challenged with BLM via the laryngotracheal route at day ( d ) 0 and d7. A 200 μL sample of saline containing 3 × 10 6 cells of hESC-MSC-IMRCs and 200 μL of hESC-MSC-IMRC-CM were delivered via the tail vein injection at d3, d7, and d14 after the first dose of BLM challenge. The lung tissues were harvested at d28 for analysis. ( b ) The effect of hESC-MSC-IMRC-CM on lung index (ratio of lung/body weights). ( c ) Representative histochemical images of H&E and Masson staining demonstrate that both hESC-MSC-IMRC-CM and hESC-MSC-IMRCs can ameliorate the pathogenesis of pulmonary fibrosis (H&E staining) with ECM deposition (Masson staining) compared to animals challenged with BLM alone. ( d ) Histological analysis of the fibrotic area. ( e , f ) The levels of hydroxyproline (Hyp) and malondialdehyde (MDA) contents. ( g ) Representative immunoblots show a reduction in indicated molecules of oxidative stress-signaling pathways and α-SMA in BLM-induced fibrotic lung administrated with hESC-MSC-IMRC-CM or hESC-MSC-IMRCs compared to the lungs of BLM-challenged mice alone. ( h ) The relative levels of Nox4, Nrf2, Ho-1 and α-SMA proteins as evaluated by a densitometric analysis in ( g ). Data in ( b , d – f , h ) represent mean ± SD of three independent experiments (N = 3), as analyzed by GraphPad prism two-way ANOVA Tukey’s multiple comparisons test. Scale bars in ( c ), 200 µm in the first and third panels, 20 µm in the second and fourth rows.

Journal: Biomedicines

Article Title: Secretome of hESC-Derived MSC-like Immune and Matrix Regulatory Cells Mitigate Pulmonary Fibrosis through Antioxidant and Anti-Inflammatory Effects

doi: 10.3390/biomedicines11020463

Figure Lengend Snippet: hESC-MSC-IMRCs and hESC-MSC-IMRC-CM inhibit the development of pulmonary fibrosis in mice. ( a ) Schematic illustration shows the experimental workflow for the treatment of the BLM-induced IPF mouse model at an early stage of PF development. C57BL/6 mice were challenged with BLM via the laryngotracheal route at day ( d ) 0 and d7. A 200 μL sample of saline containing 3 × 10 6 cells of hESC-MSC-IMRCs and 200 μL of hESC-MSC-IMRC-CM were delivered via the tail vein injection at d3, d7, and d14 after the first dose of BLM challenge. The lung tissues were harvested at d28 for analysis. ( b ) The effect of hESC-MSC-IMRC-CM on lung index (ratio of lung/body weights). ( c ) Representative histochemical images of H&E and Masson staining demonstrate that both hESC-MSC-IMRC-CM and hESC-MSC-IMRCs can ameliorate the pathogenesis of pulmonary fibrosis (H&E staining) with ECM deposition (Masson staining) compared to animals challenged with BLM alone. ( d ) Histological analysis of the fibrotic area. ( e , f ) The levels of hydroxyproline (Hyp) and malondialdehyde (MDA) contents. ( g ) Representative immunoblots show a reduction in indicated molecules of oxidative stress-signaling pathways and α-SMA in BLM-induced fibrotic lung administrated with hESC-MSC-IMRC-CM or hESC-MSC-IMRCs compared to the lungs of BLM-challenged mice alone. ( h ) The relative levels of Nox4, Nrf2, Ho-1 and α-SMA proteins as evaluated by a densitometric analysis in ( g ). Data in ( b , d – f , h ) represent mean ± SD of three independent experiments (N = 3), as analyzed by GraphPad prism two-way ANOVA Tukey’s multiple comparisons test. Scale bars in ( c ), 200 µm in the first and third panels, 20 µm in the second and fourth rows.

Techniques: Saline, Injection, Staining, Western Blot, Protein-Protein interactions

hESC-MSC-IMRC-CM alleviates the progression of pulmonary fibrosis in mice. ( a ) Schematic illustration shows the experimental workflow for treatments of late-stage of PF disease in the BLM-induced PF mouse model. C57BL/6 mice were challenged with BLM via the laryngotracheal route at day ( d ) 0 and d7. A 200 μL sample of saline containing 3 × 10 6 cells of hESC-MSC-IMRCs or 200 μL hESC-MSC-IMRC-CM was administrated via tail vein injection at d7, d14, and d21 after the first dose of BLM challenge. The lung tissues were harvested at d28 for analysis. ( b ) The ratio of lung weight/body weight (LW/BW). ( c ) Representative histochemical images of H&E and Masson staining demonstrate that both hESC-MSC-IMRC-CM and hESC-MSC-IMRCs can ameliorate the progression of pulmonary fibrosis (H&E staining) with ECM deposition (Masson staining) compared to the BLM-group. ( d ) Histological analysis of the fibrotic area. ( e , f ) The level of hydroxyproline (Hyp) and malondialdehyde (MDA) contents. ( g ) Representative immunoblots show a decreased expression of indicated components of oxidative stress signaling cascade and α-SMA in BLM-induced lung treated with hESC-MSC-IMRC-CM or hESC-MSC-IMRCs compared to the untreated BLM group. ( h ) The relative levels of Nox4, Nrf2, Ho-1, and α-SMA proteins as evaluated by a densitometric analysis in ( g ). Data in ( b , d – f , h ) represent mean ± SD of three independent experiments (N = 3), as analyzed by GraphPad prism two-way ANOVA Tukey’s multiple comparisons test. Scale bars in ( c ), 200 µm in the first and third rows, 20 µm in the second and fourth rows.

Journal: Biomedicines

Article Title: Secretome of hESC-Derived MSC-like Immune and Matrix Regulatory Cells Mitigate Pulmonary Fibrosis through Antioxidant and Anti-Inflammatory Effects

doi: 10.3390/biomedicines11020463

Figure Lengend Snippet: hESC-MSC-IMRC-CM alleviates the progression of pulmonary fibrosis in mice. ( a ) Schematic illustration shows the experimental workflow for treatments of late-stage of PF disease in the BLM-induced PF mouse model. C57BL/6 mice were challenged with BLM via the laryngotracheal route at day ( d ) 0 and d7. A 200 μL sample of saline containing 3 × 10 6 cells of hESC-MSC-IMRCs or 200 μL hESC-MSC-IMRC-CM was administrated via tail vein injection at d7, d14, and d21 after the first dose of BLM challenge. The lung tissues were harvested at d28 for analysis. ( b ) The ratio of lung weight/body weight (LW/BW). ( c ) Representative histochemical images of H&E and Masson staining demonstrate that both hESC-MSC-IMRC-CM and hESC-MSC-IMRCs can ameliorate the progression of pulmonary fibrosis (H&E staining) with ECM deposition (Masson staining) compared to the BLM-group. ( d ) Histological analysis of the fibrotic area. ( e , f ) The level of hydroxyproline (Hyp) and malondialdehyde (MDA) contents. ( g ) Representative immunoblots show a decreased expression of indicated components of oxidative stress signaling cascade and α-SMA in BLM-induced lung treated with hESC-MSC-IMRC-CM or hESC-MSC-IMRCs compared to the untreated BLM group. ( h ) The relative levels of Nox4, Nrf2, Ho-1, and α-SMA proteins as evaluated by a densitometric analysis in ( g ). Data in ( b , d – f , h ) represent mean ± SD of three independent experiments (N = 3), as analyzed by GraphPad prism two-way ANOVA Tukey’s multiple comparisons test. Scale bars in ( c ), 200 µm in the first and third rows, 20 µm in the second and fourth rows.

Techniques: Saline, Injection, Staining, Western Blot, Expressing

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